Effect of Plant Growth-Promoting Bacteria and Sulfur on growth and Micronutrient Concentration in Wheat Grain in saline-sodic Soils

Document Type : Research Paper

Authors

1 Department of Soil Science, Khuzestan Science and Research Branch, Islamic Azad University

2 Soil and Water Research Institute, Agricultural Research, Education and Extension Organization (AREEO), Karaj, Iran

3 Department of Soil Science, Ahvaz Branch, Islamic Azad University

4 Agricultural and Natural Resources Research and Education center of Khuzestan, Agricultural Research, Education and Extension Organization

10.22092/sbj.2024.356053.225

Abstract

Soil salinity and sodicity disrupt the balance of nutrients in the soil and create restrictions on plant growth. An experiment was conducted to evaluate the application of sulfur along with Thiobacillus bacteria and plant growth-promoting bacteria isolated from saline-sodic soils on the performance and concentration of micronutrients in wheat (Chamran cultivar) in saline-sodic soils in a factorial design within a completely randomized design, in three replications in greenhouse condition. The experimental factors included three types of saline-sodic soil (S1: SAR=13 and EC=8 dS m^-1), (S2: SAR=15 and EC=10 dS m^-1), and (S3: SAR=17 and EC=14 dS m^-1), four levels of plant growth-promoting bacteria (B0: control, Pseudomonas alcaliphila, Rhizobium pusense, and Bacillus subtilis) and two levels of sulfur along with Thiobacillus thiooxidans bacteria (T0: no application and T1: application of 31.4 grams per pot (10 tons per hectare) of powdered sulfur along with T. thiooxidans bacteria). Based on the sequencing of the 16S rRNA gene, the superior plant growth-promoting bacteria were identified as Pseudomonas alcaliphila, Bacillus subtilis, and Rhizobium pusense. The results showed that adding sulfur along with T. thiooxidans bacteria and other plant growth-promoting bacteria at different levels of salinity and sodicity led to an increase in grain yield and concentration of micronutrients compared to the control. At the salinity and sodicity levels of S1 and S3, the highest grain yield was observed in plants inoculated with R. pusense bacteria (13.1% and 88.8%, respectively). The combination of plant growth-promoting bacteria and sulfur along with T. thiooxidans bacteria did not have a significant effect on grain yield and concentration of micronutrients in saline-sodic soils. Overall, the individual application of R. pusense bacteria isolated from saline-sodic soils and sulfur along with T. thiooxidans bacteria plays a significant role in improving the performance and concentration of micronutrients in wheat in saline-sodic soils.

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References

  1. Abbasi, M.K., Sharif, S., Kazmi, M., Sultan, T., and Aslam, M. 2011. Isolation of plant growth promoting rhizobacteria from wheat rhizosphere and their effect on improving growth, yield and nutrient uptake of plants. Plant Biosyst. 145: 159-168.
  2. Ahmed, K., Qadir, Gh., Jami, A., Saqib, A. I. and Qaisar, M. 2017. Comparative reclamation efficiency of gypsum and sulfur for improvement of salt affected. Bulgarian Journal of Agricultural Science, 23:126-133.
  3. Alexander D, Zuberer D. 1991. Use of chrome azurol S reagents to evaluate siderophore production by rhizosphere bacteria. Biol Fertil Soils 12:39-45
  4. Alexander, D., and Zuberer, D. 1991. Use of chrome azurol S reagents to evaluate siderophore production by rhizosphere bacteria. Biol. Fertil. Soils 12: 39-45.
  5. Ao-Lei, A., Shu-Qi, N., Qi, Z., Yong-Sheng, L., Jing-Yi, G., Hui-Juan, G., Sheng-Zhou, S. and Jin-Lin, Z. 2018. Induced salt tolerance of perennial ryegrass by a novel bacterium strain from the rhizosphere of a desert shrub Haloxylon ammodendron. International Journal of Molecular Sciences, 19 (2): 469.
  6. Aria, M.M., Lakzian, A., Haghnia, G.H., Berenji, A.R., Besharati, H. and Fotovat, A. 2010. Effect of Thiobacillus, sulfur, and vermicompost on the water-soluble phosphorus of hard rock phosphate. Bioresource Technology, 101:551-554.‏
  7. Asadi Rahmani, H., Khavazi, K., Jahandideh Mahjen Abadi, V.A., Ramezanpour, M.R., Mirzapour, M.H. and Mirzashahi, K. 2018. Effect of Thiobacillus, sulfur, and phosphorus on the yield and nutrient uptake of canola and the chemical properties of calcareous soils in Iran. Communications in Soil Science and Plant Analysis, 49:1671-1683.‏
  8. Bent, E. Tuzan, S. Chanway, C P. Enebak, S, Alteration in plant growth and in root hormone levels of lodgepole pines inoculated with rhizobacteria, Canadian Journal of Microbiology, 47, PP. 793-800, 2001.
  9. Cottenie, A. 1980. Soil and plant testing as a basic fertilizer recommendation. FAO Soil Bulletin 33/2. Food and Agriculture Organization of the United Nations, Rome, Italy.
  10. Damodaran T, Mishra VK, Jha SK, Pankaj U, Gupta G, Gopal R. 2019. Identification of rhizosphere bacterial diversity with promising salt tolerance, PGP traits and their exploitation for seed germination enhancement in sodic soil. Agri Res 8:36-43.
  11. Das, P., Khan, S., Chaudhary, A.K., AbdulQuadir, M., Thaher, M.I., and Al-Jabri, H. 2019. Potential applications of algae-based bio-fertilizer. p. 41-65. In: Biofertilizers for Sustainable Agriculture and Environment. Springer, Cham.‏
  12. Das, R., Pradhan, M., Sahoo, R.K., Mohanty, D. and Kumar, M. 2021. Isolation, identification and role of novel endosymbiotic bacterium Rhizobium pusence in root nodule of Green Gram cv. OUM-11-15 (Vigna radiata) under salinity stress. Legume Research, 44: 1512-1520.‏
  13. Day, S.J., Norton, J.B., Strom, C.F., Kelleners, T.J. and Aboukila, E.F. 2018. Gypsum, langbeinite, sulfur, and compost for reclamation of drastically disturbed calcareous saline–sodic soils. International Journal of Environmental Science and Technology, 16:295-304.
  14. Dell-Amico, E., Cavalca, L., and Andreoni, V. 2005. Analysis of rhizobacterial communities in perennial Graminaceae from polluted water meadow soil, and screening of metal-resistant, potentially plant growth-promoting bacteria. FEMS Microbiol. Ecol. 52: 153–162.
  15. Elgharably A, Nafady NA (2021) Inoculation with Arbuscular mycorrhizae, Penicillium funiculosum and Fusarium oxysporum enhanced wheat growth and nutrient uptake in the saline soil. Rhizosphere 18:100345
  16. Etesami, H., and Maheshwari, D.K. 2018. Use of plant growth promoting rhizobacteria (PGPRs) with multiple plant growth promoting traits in stress agriculture: Action mechanisms and future prospects. Ecotoxicology and Environmental Safety, 156:225-246.‏
  17. Flores‐Vargas, R.D., and O'hara, W. 2006. Isolation and characterization of rhizosphere bacteria with potential for biological control of weeds in vineyards. J. Appl. Microbiol. 100: 946-954.
  18. Gee, G.W. and Bauder, J.W. 1986. Particle-size analysis. In "Methods of soil analysis, Part 1, 2nd edn" (A. Klute, ed.), pp. 383-411. Soil Science Society of America, Madison.
  19. Gouda, S., George Kerry, R., Das, G., Paramithiotis, S., Shin, H.S. and Kumar Patra, J. 2018. Revitalization of plant growth promoting rhizobacteria for sustainable development in agriculture. Microbiological Research, 26: 131-140.
  20. Gul, S., Javed, S., Azeem, M., Aftab, A., Anwaar, N., Mehmood, T. and Zeshan, B. 2023. Application of Bacillus subtilis for the alleviation of salinity stress in different cultivars of wheat (Tritium aestivum). Agronomy: 13:, 437.‏
  21. Hamonts, K., Trivedi, P., Garg, A., Janitz, C., Grinyer, J., Holford, P., Botha, F., Anderson, I.C. and Singh, B.K. 2018. Field study reveals core plant microbiota and relative importance of their drivers. Environmental Microbiology, 20:124-140.‏
  22. Han, H.S. and Lee, K.D. 2005. Physiological responses of soybean-inoculation of Bradyrhizobium japonicum with PGPR in saline soil conditions. Research Journal of Agriculture and Biological Sciences. 1: 216-221.‏
  23. Helmke, P.A. and Sparks, D.L. 1996. Lithium, sodium, potassium, rubidium, and cesium. Methods of soil analysis: Part 3 chemical methods. 5:551-574.
  24. Huber, M.V., Bienvenut, W., Linster, E., Stephan, I., Armbruster, L., Sticht, C., Layer, D. and Lapouge, K. 2020. NatB-mediated n-terminal acetylation affects growth and biotic stress responses. Plant Physiology, 182:792-806.
  25. Jiang, H., Dong, H., Zhang, G., Yu, B., Chapman, L.R., and Fields, M.W. 2006. Microbial diversity in water and sediment of Lake Chaka, an athalassohaline lake in northwestern China. Applied and Environmental Microbiology, 72: 3832-3845.‏
  26. Khavazi, K, Jahandideh Mahjan Abadi, V.A., Taghipoor, F. 2018. Effect of Sulfur, Thiobacillus bacteria and phosphorus on the yield and nutrient elements uptake of wheat in calcareous soil. Journal of Soil Management and Sustainable Productio, 8:23-41.
  27. Kumar Arora, N., Tahmish, F., Mishra, I., Verma, M. and Mishra, J. 2018. Environmental sustainability: challenges and viable solutions. Environmental Sustainability, 1:309-340.
  28. Mahdy, A.M. 2011. Comparative effects of different soil amendments on amelioration of saline-sodic soils. Soil and Water Research, 6:205-216.‏
  29. Mane, A.V., Deshpande, T.V., Wagh, V.B., Karadge, B.A. and Samant, J.S. 2011. A critical review on physiological changes associated with reference to salinity. International Journal of Environmental Sciences, 6:1192-1216.
  30. Manesh, A.K., Armin, M. and Moeini, M.J. 2013. The effect of sulfur application on yield and yield components of corn in two different planting methods in saline conditions. International Journal of Plant Production, 4:1474-1478.
  31. Maxton, A., Singh, P. and Masih, S.A. 2018. ACC deaminase-producing bacteria mediated drought and salt tolerance in Capsicum annuum. Journal of Plant Nutrition, 41:574-583.‏
  32. Naseem, H., Ahsan, M., Shahid, M.A. and Khan, N. 2018. Exopolysaccharides producing rhizobacteria and their role in plant growth and drought tolerance. Journal of Basic Microbiology, 58:1009-1022.‏
  33. Nawaz, A., Shahbaz, M., Asadullah Imran, A., Marghoob, M.U., Imtiaz, M. and Mubeen, F. 2020. Potential of salt tolerant PGPR in growth and yield augmentation of wheat (Triticum aestivum) under saline conditions. Frontiers in Microbiology, 11: 457-475.
  34. Olsen, S. R. 1954. "Estimation of available phosphorus in soils by extraction with sodium bicarbonate," United States Department Of Agriculture; Washington.
  35. Rashid, M.S., Khalil, N., Ayub, S., Alam, S. and Latif, F. 2004. Organic acids production and phosphate solubilization by phosphate solubilizing microorganisms (PSM) under in vitro conditions. Pakistan Journal of Biological Sciences. 7, 187-196.
  36. Ryan, J., Estefan, G. and Rashid, A. 2001. Soil and plant analysis: laboratory ICARDA, Aleppo.
  37. Sambrook, J., and Russell, D.W. 2001. Molecular Cloning. A laboratory manual. Cold Spring Harbor Laboratory Press, New York.
  38. Sandhya, V., and Ali, S.Z. 2015. The production of exopolysaccharide by Pseudomonas putida GAP-P45 under various abiotic stress conditions and its role in soil aggregation. Microbiology 84:512–519
  39. Sharma, A., Dev, K., Sourirajan, A. and Choudhary, M. 2021. Isolation and characterization of salt-tolerant bacteria with plant growth-promoting activities from saline agricultural fields of Haryana, India. Journal of Genetic Engineering and Biotechnology, 19:1-10
  40. Suarez, D.L. 1996. Beryllium, magnesium, calcium, strontium, and barium. Methods of soil analysis: Part 3 chemical methods 5:575-601.
  41. Tamura, K., Dudley, J., Nei, M. and Kumar, S. 2007. MEGA4: molecular evolutionary genetics analysis (MEGA) software version 4.0. Molecular Biology and Evolution, 24:1596-1599.
  42. Ullah, A., Bano, A. 2019. Role of PGPR in the reclamation and revegetation of saline land. Pakistan Journal of Botany, 51:27–35
  43. Vejan, P., Abdullah, R., Khadiran, T., Ismail, S. and Nasrulhaq Boyce, A. 2016. Role of plant growth promoting rhizobacteria in agricultural sustainability—a review. Molecules, 21:573.‏
  44. Vurukonda, S.S.K.P., Vardharajula, S., Shrivastava, M. and SkZ, A. 2016. Enhancement of drought stress tolerance in crops by plant growth promoting rhizobacteria. Microbiological Research, 184: 13-24.‏
  45. Walkley, A., and Black, I.A. 1934. An examination of the degtjareff method for determining soil organic matter, and a proposed modification of the chromic acid titration method. Soil Science, 37: 29-38.
  46. Yıldıztekin, M. and Kuzu, S. 2019. Soil properties and mineral nutrients of clementine mandarine (Citrus reticulata Blanco) grown in the Koycegiz region of Mugla province. International Journal of Secondary Metabolite, 6:323-332.
  47. Zafar-ul-Hye, M., Nasir, A., Aon, M., Hussain, S., Ahmad, M. and Naz, I. 2018. Seed inoculation with Pseudomonas fluorescens and Pseudomonas syringae enhanced maize growth in a compacted saline-sodic soil. Phyton, 87: 25-31.
  48. Zeng, Q., Man, X., Huang, Z., Zhuang, L., Yang, H. and Sha, Y. 2023. Effects of rice blast biocontrol strain Pseudomonas alcaliphila Ej2 on the endophytic microbiome and proteome of rice under salt stress. Frontiers in Microbiology, 14: 1129614.‏
  49. Zulueta-Rodriguez, R., Cordoba-Matson, M.V., Hernandez-Montiel, L.G., Murillo-Amador, B., Rueda-Puente, E. and Lara, L. 2014. Effect of Pseudomonas putida on growth and anthocyanin pigment in two poinsettia (Euphorbia pulcherrima) cultivars. The Scientific World Journal, 810192.‏
Journal of Sol Biology
Volume 11, Issue 2
March 2024
Pages 167-182

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