اثر رشد گلدانی پنبه تراریخته Bt روی باکتری‌های ریزوسفری و غیرریزوسفری خاک

نوع مقاله: مقاله پژوهشی

نویسندگان

1 دانشجوی دکتری دانشگاه فردوسی مشهد

2 دانشیار پژوهشکده بیوتکنولوژی کشاورزی ایران، دانشکده مهندسی انرژی و فناوری‌های نوین، دانشگاه شهید بهشتی

3 استاد دانشگاه فردوسی مشهد

4 دانشیار دانشگاه فردوسی مشهد

5 مربی پژوهشکده بیوتکنولوژی کشاورزی ایران

چکیده

در این مطالعه، پنبه تراریخته  Bacillus thuringiensis (Bt)نسل T2 (لاین 61) و پنبه غیرتراریخته برای بررسی پویایی باکتری‌ها در طی دوره‌های رشد و نمو ( نونهالی، تشکیل کاسبرگ، گلدهی و تشکیل غوزه) با استفاده از دو روش قابل کشت (شمارش کلنی‌های زنده) و غیرقابل کشتPCR-DGGE  (polymerase chain reaction-denaturing gradient gel electrophoresis) مورد ارزیابی قرار گرفت. تعداد باکتری‌هایعملکردی تثبیت‌کننده نیتروژن، حل‌کننده فسفات معدنی و آلی ناحیه ریزوسفر از طریق کشت در محیط‌های ویژه رشد باکتری تعیین شد. آنالیز شمارش کلونی‌های زنده، افزایش در ساختار جمعیت و تعداد کل باکتری‌های ریزوسفر را در هر دو واریته تراریخته و غیرتراریخته در مقایسه با خاک غیرریزوسفری را نشان داد. روش PCR-DGGE موید نتایج ارزیابی شمارش کلونی‌های زنده بود که تغییرات در جمعیت‌های باکتری‌ها برای تمام مراحل رشدی پنبه در خاک ریزوسفر و غیرریزوسفری را نشان می‌داد. تعداد باکتری‌های عملکردی میان پنبه تراریخته Bt و پنبه غیرتراریخته در یک مرحله مشخص اختلاف معنی‌دار نداشت. با این وجود اختلاف معنی‌دار در تعداد جمعیت باکتری‌های ریزوسفر خاک در مراحل مختلف رشدی در هر دو گیاه تراریخته و غیرتراریخته مشاهده شد ولی برای خاک غیرریزوسفری مشاهده نشد. در مجموع نتایج نشان داد، پنبه تراریخته Bt نسل T2 (لاین 61) احتمال وجود اثرات نامطلوب روی ساختار جمعیت و تعداد کل باکتری‌های قابل کشت در ناحیه ریزوسفر را نداشت. 

کلیدواژه‌ها


عنوان مقاله [English]

Effect of Bt-transgenic cotton pot cultivation on rhizosphere and non-rhizosphere bacterial community

نویسندگان [English]

  • Z. S. Shahmoradi 1
  • M. Tohidfar 2
  • H. Marashi 3
  • S. Malekzadeh-shafaroudi 4
  • E. Karimi 5
1 PhD student of Ferdowsi University of Mashhad
2 Associate professor, Agricultural Biotechnology Research Institute of Iran, Faculty of Energy Engineering and New Technology, Shahid Beheshti University
3 Professor, Ferdowsi University of Mashhad
4 Associate professor, Ferdowsi University of Mashhad
5 Instructor, Agricultural Biotechnology Research Institute of Iran (ABRII)
چکیده [English]

In this study, effects of T2 Bacillus thuringiensis (Bt) transgenic cotton (line 61) and its non-transgenic line on bacterial dynamics were investigated during the cotton development stages (seedling, squaring, flowering and boll formation) using culturable (viable plate count) and unculturabale (polymerase chain reaction-denaturing gradient gel electrophoresis, PCR-DGGE) assays. The number nitrogen-fixing bacteria, organic and inorganic phosphate-dissolving bacteria in the rhizosphere soil were also determined by cultivation on specific growth media. Viable plate count analysis showed an increase in the number of total bacteria in rhizosphere soils of both transgenic and non-transgenic cultivars as compared to the bulk soil. PCR-DGGE confirmed the results of viable plate count assays. No significant differences in number of functional bacteria were observed between rhizosphere soil of Bt transgenic and non-transgenic cotton at one particular stage. However, significant differences in bacterial population size in rhizosphere soil observed during the cotton development stages in both transgenic and non-transgenic plants, but not for bulk soil. The results indicated that Bt transgenic cotton (line 61) might have no adverse effects on community structures and total number of culturable bacteria in the rhizosphere.

کلیدواژه‌ها [English]

  • DGGE
  • Microbial communities
  • Rhizosphere soil
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