تغییرات فصلی فعالیت مطلق و ویژه‌ی اسیدفسفاتاز در رقابت ریزوسفری نهال‌های بلندمازو و پلت در کشت خالص و آمیخته

نوع مقاله: مقاله پژوهشی

نویسندگان

1 دانش‌آموخته دکتری دانشگاه علوم کشاورزی و منابع طبیعی گرگان

2 دانشیار دانشگاه علوم کشاورزی و منابع طبیعی گرگان

3 استادیار دانشگاه علوم کشاورزی و منابع طبیعی گرگان

چکیده

رقابت ریزوسفری در دستیابی گیاهان به عناصر غذایی مانند فسفر اهمیت زیادی دارد. محیط ریزوسفر حداکثر اثرپذیری را از رقابت بین‌گونه‌ای و درون‌گونه‌ای دارد. با توجه به ویژگی‌های ریزوسفری خاص هر گونه احتمالاً تراوش‌های ریزوسفری (مانند اسیدفسفاتاز) در حالات رقابتی مختلف، تغییرات محسوسی دارد. اسیدفسفاتاز از مهم‌ترین آنزیم‌های ریزوسفری و آنزیم مختص چرخه‌ی فسفر است. این پژوهش به مطالعه‌ی‌ فعالیت مطلق و ویژه‌ی ‌اسیدفسفاتاز در ریزوسفر دو گونه‌‌ی پلت و بلندمازو (از مهم‌ترین گونه‌های جنگلی شمال کشور) در حالت کشت خالص و آمیخته در طی فصول مختلف در یک ریزوترون نوین پرداخته است. از لوله‌های دستیابی 18 ریزوترون شامل سه تیمار آمیختگی (پلت خالص، بلندمازو خالص و پلت- بلندمازو) درپنج تکرار همراه با سه ریزوترون فاقد کشت نهال طی چهار فصل سال نمونه‌گیری انجام و فعالیت مطلق و ویژه‌ی اسیدفسفاتاز در آن تعیین گردید. نتایج نشان داد که فعالیت مطلق و ویژه‌‌ی اسیدفسفاتاز بین ترکیب‌های متفاوت کشت و فصول مختلف در سطح احتمال 95 درصد اختلاف معنی‌داری دارد. ترکیب کشت آمیخته در پاییز بیشترین میزان فعالیت (gPNPg-1h-1µ 16/154±37/2370) و تیمار کنترل در تابستان کمترین میزان فعالیت اسیدفسفاتاز مطلق (µgPNPg-1h-1 65/108±75/991) را به خود اختصاص دادند. میزان فعالیت مطلق و ویژه‌ی ‌اسیدفسفاتاز در انتهای دوره‌‌ی مطالعه نسبت به فصل آغازین مطالعه کاهش محسوسی را نشان داد. این نتایج می‌تواند تأییدکننده‌ی‌ برتری کشت آمیخته‌ی پلت- بلندمازو از نظر ساختار، فیزیولوژی و مورفولوژی مناسب‌تر ریزوسفری و رشد بهتر ریشه باشد و این تیمار، فسفر خاک را در طی فصول مختلف به میزان بهتری حفظ نموده است.

کلیدواژه‌ها


عنوان مقاله [English]

Seasonal Variations of Absolute and Specific Acid Phosphatase Activities on Rhizosphere Competition in Pure and Mixed Planting of Chestnut-Leaved Oak and Persian Maple

نویسندگان [English]

  • Y. Sharifpour 1
  • H. Habashi 2
  • A. Aliarab 3
1 Ph. D. Graduate, Gorgan University of Agricultural Science and Natural Resource
2 Associate professor, Gorgan University of Agricultural Science and Natural Resource
3 Assistant professor, Gorgan University of Agricultural Science and Natural Resource
چکیده [English]

Rhizospheric competition play a vital role in plant nutrition such as phosphorus availability. Inter and intraspecific competition have the maximum influence on rhizosphere function. It can be expected that rhizosphere exudations (such as acid phosphatase) will change in the various competitive scenarios, considering the specific rhizosphere characteristics of plant species. Acid phosphatase (AP) is one of the most important rhizospheric enzymes and it is a specific enzyme to the phosphorus cycle. This investigation was carried out to study the absolute and specific AP activities on rhizosphere competition in pure and mixed planting of Quercus castaneifolia and Acer velutinium (two important species of forests of northern Iran) during different seasons by a novel rhizotron. Sampling was done within 18 rhizotron access tube (3 composition treatments/5 replications with 3 controls rhizotron without seedling) in 4 seasons, then absolute and specific AP activities were determined. Results revealed that there were significant differences between the absolute and the specific AP activities of different planting compositions and seasons (P<0.05). Mixed planting had the most absolute AP activities (2370.37±154.16µgPNPg-1h-1) in autumn while control treatment had the least absolute AP activities (991.75±108.65µgPNPg-1h-1) in summer. Amounts of absolute and specific AP activities showed significant decrease at the end of experiment compared with the beginning of study times. Results showed Quercus castaneifolia and Acer velutinium companion planting had better rhizosphere structure, physiology and morphology and also showed that mixed planting have better root growth, so we concluded that this treatment provide more phosphorus during different seasons.

کلیدواژه‌ها [English]

  • Acid Phosphatase
  • species composition
  • Root Competition
  • Rhizotron
  1. ثاقب‌طالبی، خ. 1378. نیاز رویشگاهی و نحوه زیست گونه افرا (پلت) Acer velutiniumBoiss.در جنگل خیرودکنار نوشهر. تحقیقات جنگل و صنوبر ایران. ج 2. ش 1. ص 79- 150.
  2. رئیسی، ش؛ جلالی، غ‌ م؛ اسپهبدی، ک. و خورنکه، س ا. 1391. بررسی تنوع در خصوصیات مورفولوژیکی برگ و میوه بلوط بلندمازو (Quercuscastaneifolia)  در 5 رویشگاه طبیعی جنگل‌های مازندران. پژوهش‌های علوم و فناوری چوب و جنگل. ج 19. ش 4. ص 93-108.
  3. Ben-Dor, E.and Banin, A. 1989. Determination of organic matter content in arid zone soils using a simple "loss-on-ignition" method. Communiction in SoilScience and Plant Analysis 20(15-16): 1675-1695.
  4. Bagodatskaya, E., Blagodatsky, S., Anderson, T.H. andKuzyakov, Y. 2009. Contrasting effects of glucose, living roots and maize straw on microbial growth kinetics and substrate availability in soil. European Journal of Soil Science 60: 186-197.
  5. Blosfeld, S., Gansert, D., Thiele, B., Kuhn, A.J., Kuhn,A.J. and Losch, R.2011.The dynamics of oxygen concentration, pH value, and organic acids in the rhizosphere of Juncusspp. Soil Biology and Biochemistry 43: 1186-1197.
  6. Bouillet, J.P., Laclau, J.P., Gonçalves, J.L.D.M, Voigtlaender, M., Gava, J.L., Leite, F.P., Hakamada, R., Mareschal, L., Tardy, F.,Levillain, J., Deleporte, P., Epron, D., Mabiala, A. and Nouvellon, Y. 2013. Eucalyptus and Acacia tree growth over entire rotation in singleand mixed-species plantations across five sites in Brazil and Congo. Forest Ecology and Management 301: 89–101.
  7. Busgen, M., Munch, E.T.and Thomsom, T. 1929.The structure and life of forest trees.Chapman and Hall, London.436 pp.
  8. Castillo, J.D., Comas, C., Voltas, J.andFerrio, J.P. 2016. Dynamics of competition over water in a mixed oak-pine Mediterranean forest: Spatio-temporal and physiological components. Forest Ecology and Management 382: 214–224.
  9. Cesarz, S., Fender, A.C., Beyer, F., Valtanen, K., Gansert, D., Hertel, D., olle, A., Daniel, R., Pfeiffer, B., Leuschner, C. and Scheu, S.2013. Roots from beech (Fagussylvatica L.) and ash (Fraxinus excelsior L.) differentially affect soil microorganisms and carbon dynamics. Soil Biology and Biochemistry 6: 123-32.
  10. Dick, W.A. andTabatabai, M.A.1992. Significance and potential uses of soil enzymes. In: Metting Jr.,.B. (Ed.), Soil Microbial Ecology: Application in Agricultural and Environmental Management. Marcel Dekker. New York. pp: 95–125.
  11. Eivazi, F.and Tabatabai, M.A.1977.Phosphatases in soils. Soil Biology and Biochemistry 9:167–172.
  12. Fender, A.C., Gansert, D., Jungkunst, H.F., Fiedler, S., Schützenmeistera, K., Thieled, B., Valtanene, K., Polleec, A., Beyer, F. and Leuschnera, C.2013. Root-induced tree species effects on the source/sink strength for greenhouse gases (CH4,N2O and CO2) of a temperate deciduous forest soil. Soil Biology and Biochemistry 57: 587-597.
  13. Fruleux, A., Bonal, D. and Bogeat-Triboulot, M.B. 2016. Interactive effects of competition and water availability on above and belowground growth and functional traits of European beech at juvenile level. Forest Ecology and Management 382: 21–30.
  14. Godin, A.M., Lidher, K.K., Whiteside, M.D. and Jones, M.D. 2015. Control of soil phosphatase activities at millimeter scales in a mixed paper birch e Douglas-fir forest: The importance of carbon and nitrogen. Soil Biology and Biochemistry 80: 62-69.
  15. Hendriksen, N.B., Creamer, R.E., Stone, D.and Winding, A. 2016. Soil exo-enzyme activities across Europe the influence of climate, land-use and soil properties. Applied Soil Ecology 97: 44–48.
  16. Herold, N., Schöning, I., Berner, D., Haslwimmer, H., Kandeler, E., Michalzik, B. and Schrumpf, M. 2014. Vertical gradients of potential enzyme activities in soil profiles of European beech, Norway spruce and Scots pine dominated forest sites. Pedobiologia 54(3): 181-189.
  17. Hou, E., Chen, C., Wen, D. andLiub, X. 2015. Phosphatase activity in relation to key litter and soil properties in mature subtropical forests in China. Science of the Total Environment 515–516: 83–91.
  18. Isaac, R.A. and Jones,J.B. 1972. Effects of various drying temperatures on the determination of five plant tissues. Communication in Soil Science and. Plant Analysis 3: 261- 269.
  19. Kotroczó, Z., Veres, Z., Fekete, I., Krakomperger, Z., Tóth, J.A., Lajtha, K. and Tóthmérész, B. 2014. Soil enzyme activity in response to long-term organic matter manipulation. Soil Biology and Biochemistry 70: 237-243.
  20. Lei, P., Scherer-Lorenzen,M.andBauhus, J.2012.Belowground facilitation and competition in young tree species mixtures. Forest Ecology and Management 265:191–200.
  21. Margesin, R. 1996. Acid and alkaline phosphomonoesterase with the substrate p-nitrophenyl phosphate [in: Methods in soil biology. Schinner F, Ohlinger E, KandelerE,Margesin R (eds.)].Berlin. Springer Verl.213–217.
  22. Marinari, S., Moscatelli, C. and Grego, S. 2014. Enzymes at plant-soil interface. In: Gianfreda, L., Rao, M.A. (Eds.), Enzymes in Agricultural Sciences. OMICS Group eBooks, USA, pp. 94 -109.
  23. Meier,I.C., Finzi, A.C. and Phillips, R.P. 2017. Root exudates increase N availability by stimulating microbial turnover of fast-cycling N pools. Soil Biology and Biochemistry 106: 119-128.
  24. Moorberg, C.J., Vepraskas, M.J. and Niewoehner, C.P. 2013. Dynamics of P dissolution processes in the matrix and rhizospheres of bald cypress growing in saturated soil. Geoderma 202–203: 153–160.
  25. Mukhopadhyay, S.and Joy, V.C. 2010. Influence of leaf litter types on microbial functions and nutrient status of soil: Ecological suitability of forest trees for afforestation in tropical laterite wastelands. Soil Biology and Biochemistry 42: 2306-2315.
  26. Pretzsch, H., Forrester, D.I.andThomasRötzer, T. 2015. Representation of species mixing in forest growth models. A review and perspective. Ecological Modelling 313: 276–292.
  27. Priyadarshini, K.V.R., Bie, S.D., Heitkonig, I.M.A., Woodborne, S., Gort, G., Kirkman, K.P. and Prins, H.H.T. 2016. Competition with trees does not influence root characteristics of perennial grasses in semi-arid and arid savannas in South Africa. Journal of Arid Environments 124: 270-277.
  28. Razavi, B.S., Zarebanadkouki, M., Blagodatskaya, E., and Kuzyakov, Y. 2016. Rhizosphere shape of lentil and maize: Spatial distribution of enzyme activities. Soil Biology and Biochemistry 96: 229-237.
  29. Schmid, I. 2002. The influence of soil type and interspecific competition on the fine root system of Norway spruce and European beech. Basic and Applied Ecology 3: 339–346.
  30. Šnajdr, J., Valášková, V., Merhautová, V., Herinková, J., Cajthaml, T. and Baldrian, P.2008. Spatial variability of enzyme activities and microbial biomass in the upper layers of Quercuspetraea forest soil. Soil Biology and Biochemistry 40: 2068–2075.
  31. Song, M., Tian, Y., Xu, X., Hu, Q. and Ouyang, H.2006. Interactions between root and shoot competition among four plant species in an alpine meadow on the Tibetan Plateau. ActaOecologica 29:214– 220.
  32. Spohn, M. and Kuzyakov, Y. 2013. Distribution of microbial- and root-derived phosphatase activities in the rhizosphere depending on P availability and C allocation - Coupling soil zymography with C14 imaging. Soil Biology and Biochemistry. 67: 106-113.
  33. Spohn, M., Carminati, A. and Kuzyakov, Y. 2013. Soil zymography: A novel in situ method for mapping distribution of enzyme activity in soil. Soil Biology and Biochemistry 58: 275-280.
  34. Tabatabai, M.A. and Bremner, J.M. 1969.Use of P-nitrophenyl phosphate for assay soil phosphatas activity. Soil Biology and Biochemistry 1(4): 307–310.
  35. Trasar-Cepeda, C., Leiro, M.C. and Gil-Sotres, F. 2008. Hydrolytic enzyme activities in agricultural and forest soils some implications for their use as indicators of soil quality.Soil Biology and Biochemistry 40: 2146–2155.
  36. Ushio, M., Kitayama, K. and Balser, T.C. 2010. Tree species effects on soil enzyme activities through effects on soil physicochemical and microbial properties in a tropical montane forest on Mt. Kinabalu, Borneo. Pedobiologia 53: 227–233.
  37. Wang, B., Xue, S., Liu, G.B., Zhang, G.H., Li, G. and Ren, Z.P. 2012.Changes in soil nutrient and enzyme activities under different vegetation in the Loess Plateau area, Northwest China. Catena 92: 186–195.
  38. Wittmann, C., Kähkönen, M.A., Ilvesniemi, H., Kurola, J. and Salkinoja-Salonen, M.S. 2004. Areal activities and stratification of hydrolytic enzymes involved in the biochemical cycles of carbon, nitrogen, sulphur and phosphorus in podsolized boreal forest soils. Soil Biology and Biochemistry 36: 425–433.
  39. Zhang, C., Liu, G.,Xue, S.and Song, Z. 2011. Rhizosphere soil microbial activity under different vegetation types on the Loess Plateau, China. Geoderma 161: 115–125.
  40. Zheng, J., Chen, J., Pan, G., Wang, G., Zhang, X., Li, L., Bian, R., Cheng, K., Liu, X.and Zheng, J. 2017. A long-term hybrid poplar plantation on cropland reduces soil organic carbon Mineralization and shifts microbial community abundance and composition. Applied Soil Ecology 111: 94–104.