اثر تنظیم pH کمپوست شهری بر غنی‌سازی آن با باکتری محرک رشد گیاه Enterobacter cloacae

نوع مقاله : مقاله پژوهشی

نویسندگان

1 دانشجوی دکترای بیولوژی و بیوتکنولوژی خاک، گروه علوم و مهندسی خاک، دانشکده کشاورزی، دانشگاه تبریز

2 استاد گروه علوم و مهندسی خاک، دانشکده کشاورزی، دانشگاه تبریز

3 دانشجوی دکترای زیست‌شناسی، گروه زیست‌شناسی، دانشکده علوم، دانشگاه تبریز

چکیده

برای افزایش کارایی و کیفیت کمپوست، غنی‌سازی عنصری و میکروبی از راهکارهای اساسی است. افزودن باکتری‌های مفید و محرک رشد گیاهان از جنبه‌های مختلف زیست‌محیطی مورد تأیید می‌باشد. از عوامل مؤثر بر مدت زندمانی باکتری در کمپوست، pH آن می‌باشد. در این تحقیق کمپوست شهری را با اتوکلاو استریل نموده و به دو بخش شامل الف- کمپوست با pH اولیه (6/5) و ب- کمپوست با pH تنظیم شده در هفت توسط کربنات کلسیم) تقسیم شد. مایه‌زنی با باکتری Enterobacter cloacae با جمعیت CFU g-1 109 انجام گرفت. رطوبت کمپوست در حدود 40 درصد ظرفیت نگهداری آب تنظیم و نمونه‌ها در انکوباتور با دمای 26 درجه سلسیوس نگهداری شدند. شمارش جمعیت میکروبی و اندازه‌گیری pH کمپوست در زمان‌های 3، 15، 45، 75، 105، 135 ، 165 و 195روز پس از تلقیح انجام گردید. نتایج شمارش جمعیت میکروبی نشان داد که اثر pH در زنده‌مانی باکتری در کمپوست معنی‌دار بود (p<0.01). 45 روز پس از تلقیح، جمعیت میکروبی در کمپوست باpH  تنظیم نشده به حدود  CFU g-1  104 تقلیل یافت در حالی که در کمپوست تنظیم شده از نظر pH، این تعداد برابر  CFU g-1  109 بود. علیرغم نوسانات جمعیت میکروبی در کمپوست تنظیم نشده، تا 135 روز بعد از تلقیح در هر دو کمپوست جمعیت CFU g-1  107 حاکم بود. تعداد جمعیت باکتری در کمپوست تنظیم شده بعد از 165 روز و کمپوست بدون تنظیم بعد از 140 روز به جمعیت زیر حد استاندارد (CFU g-1  106) رسیدند. 

کلیدواژه‌ها


عنوان مقاله [English]

The Effect of pH Adjustment of Municipal Compost on its Enrichment with Plant Growth Promoting Bacterium "Enterobacter Cloacae"

نویسندگان [English]

  • B. Khoshru 1
  • N. Aliasgharzad 2
  • A. Jodmand 3
1 PhD Student of Soil Biology and Biotechnology, Faculty of Agriculture, Univ. of Tabriz, Iran
2 Professor of Soil Biology and Biotechnology, Dept. of Soil Science, Faculty of Agriculture, University of Tabriz, Iran
3 PhD Student of Biology, Faculty of Science, University. of Tabriz Iran
چکیده [English]

Elemental and microbial enrichment of compost are basic solutions to increase its quality. The addition of beneficial bacteria and plant growth promoters are confirmed by various environmental aspects. The pH of compost is a critical factor affecting bacterial persistence. In this study, the municipal compost was sterilized and divided into two parts: compost with initial pH (5.6) and compost with pH adjusted to 7 by calcium carbonate addition. Incubation of compost with Enterobacter cloacae was performed with 109 CFU g-1 population. The moisture content of the compost adjusted to about 40% of the water holding capacity and the samples were kept in an incubator at 26 °C. The microbial counting and pH measurement were accomplished at 3, 15, 45, 75, 105, 135, 165 and 195 days after inoculation. The results of microbial counting showed that the effect of pH on bacterial viability in the compost was significant (p<0.01). Forty-five days after inoculation, the microbial count in non-amended compost declined to about 104 CFU g-1 while in the amended compost it was 109 CFU g-1. Regardless of the fluctuations of microbial population in non-amended compost, the bacterial population was 107 CFU g-1 in both divisions of compost after 135 days' incubation. The number of bacteria in amended and non-amended composts was below the standard limit (106 CFU g-1) after 165 and 140 days incubation, respectively. 

کلیدواژه‌ها [English]

  • Compost
  • microbial enrichment
  • phosphate solubilizing bacteria
  • pH
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