اثر تنظیم pH کمپوست شهری بر غنی‌سازی آن با باکتری محرک رشد گیاه Enterobacter cloacae

نوع مقاله: مقاله پژوهشی

نویسندگان

1 دانشجوی دکترای بیولوژی و بیوتکنولوژی خاک، گروه علوم و مهندسی خاک، دانشکده کشاورزی، دانشگاه تبریز

2 استاد گروه علوم و مهندسی خاک، دانشکده کشاورزی، دانشگاه تبریز

3 دانشجوی دکترای زیست‌شناسی، گروه زیست‌شناسی، دانشکده علوم، دانشگاه تبریز

چکیده

برای افزایش کارایی و کیفیت کمپوست، غنی‌سازی عنصری و میکروبی از راهکارهای اساسی است. افزودن باکتری‌های مفید و محرک رشد گیاهان از جنبه‌های مختلف زیست‌محیطی مورد تأیید می‌باشد. از عوامل مؤثر بر مدت زندمانی باکتری در کمپوست، pH آن می‌باشد. در این تحقیق کمپوست شهری را با اتوکلاو استریل نموده و به دو بخش شامل الف- کمپوست با pH اولیه (6/5) و ب- کمپوست با pH تنظیم شده در هفت توسط کربنات کلسیم) تقسیم شد. مایه‌زنی با باکتری Enterobacter cloacae با جمعیت CFU g-1 109 انجام گرفت. رطوبت کمپوست در حدود 40 درصد ظرفیت نگهداری آب تنظیم و نمونه‌ها در انکوباتور با دمای 26 درجه سلسیوس نگهداری شدند. شمارش جمعیت میکروبی و اندازه‌گیری pH کمپوست در زمان‌های 3، 15، 45، 75، 105، 135 ، 165 و 195روز پس از تلقیح انجام گردید. نتایج شمارش جمعیت میکروبی نشان داد که اثر pH در زنده‌مانی باکتری در کمپوست معنی‌دار بود (p<0.01). 45 روز پس از تلقیح، جمعیت میکروبی در کمپوست باpH  تنظیم نشده به حدود  CFU g-1  104 تقلیل یافت در حالی که در کمپوست تنظیم شده از نظر pH، این تعداد برابر  CFU g-1  109 بود. علیرغم نوسانات جمعیت میکروبی در کمپوست تنظیم نشده، تا 135 روز بعد از تلقیح در هر دو کمپوست جمعیت CFU g-1  107 حاکم بود. تعداد جمعیت باکتری در کمپوست تنظیم شده بعد از 165 روز و کمپوست بدون تنظیم بعد از 140 روز به جمعیت زیر حد استاندارد (CFU g-1  106) رسیدند. 

کلیدواژه‌ها


عنوان مقاله [English]

The Effect of pH Adjustment of Municipal Compost on its Enrichment with Plant Growth Promoting Bacterium "Enterobacter Cloacae"

نویسندگان [English]

  • B. Khoshru 1
  • N. Aliasgharzad 2
  • A. Jodmand 3
1 PhD Student of Soil Biology and Biotechnology, Faculty of Agriculture, Univ. of Tabriz, Iran
2 Professor of Soil Biology and Biotechnology, Dept. of Soil Science, Faculty of Agriculture, University of Tabriz, Iran
3 PhD Student of Biology, Faculty of Science, University. of Tabriz Iran
چکیده [English]

Elemental and microbial enrichment of compost are basic solutions to increase its quality. The addition of beneficial bacteria and plant growth promoters are confirmed by various environmental aspects. The pH of compost is a critical factor affecting bacterial persistence. In this study, the municipal compost was sterilized and divided into two parts: compost with initial pH (5.6) and compost with pH adjusted to 7 by calcium carbonate addition. Incubation of compost with Enterobacter cloacae was performed with 109 CFU g-1 population. The moisture content of the compost adjusted to about 40% of the water holding capacity and the samples were kept in an incubator at 26 °C. The microbial counting and pH measurement were accomplished at 3, 15, 45, 75, 105, 135, 165 and 195 days after inoculation. The results of microbial counting showed that the effect of pH on bacterial viability in the compost was significant (p<0.01). Forty-five days after inoculation, the microbial count in non-amended compost declined to about 104 CFU g-1 while in the amended compost it was 109 CFU g-1. Regardless of the fluctuations of microbial population in non-amended compost, the bacterial population was 107 CFU g-1 in both divisions of compost after 135 days' incubation. The number of bacteria in amended and non-amended composts was below the standard limit (106 CFU g-1) after 165 and 140 days incubation, respectively. 

کلیدواژه‌ها [English]

  • Compost
  • microbial enrichment
  • phosphate solubilizing bacteria
  • pH
  1. Aliasgharzad, N. 1997. Soil Microbiology and Biochemistry (Farsi translation). First Edition. Tabriz University Press.
  2. Allen, M. J., Edberg, S.C. and Reasoner, D. J. 2004. Heterotrophic plate count bacteria what is their significance in drinking water. International Journal of Food Microbiology, 92: 265-274.
  3. Adani, F., Genevini, P.L. and Tambone, F. 1995. A new index of organic matter stability. Compost science & utilization 3: 25-37.
  4. Azarmi, R., Sharifi, Z. and Satari, M.R. 2008. Effect of vermicompost on growth, yield and nutrition status of tomato (Lycopersicum esculentum). Pakistan Journal of Biological Sciences 11: 1797-1802.
  5. Beauchamp, C.J., Levesque, G., Prevost, D. and Chalifour, F.P. 2006. Isolation of free-living dinitrogen fixing bacteria and their activity in compost containing de-inking paper sludge. Bioresource Technology 97: 1002–1011.
  6. Combet-Blanc, Y., Kalamba, K.K. and Kergoat, P.Y. 1995. Effect of pH on Bacillus thermoamylovorans growth and glucose fermentation. Applied and Environmental Microbiology 61: 651–659
  7. Desai, J.D. and Banat, I.M. 1997. Microbial production of surfactants and their commercial potential. Microbiology and molecular biology reviews 61: 47-64.
  8. Edwards, C.A., Domínguez, J. and Arancon, N.Q. 2004. The influence of vermicomposts on plant growth and pest incidence. In Shakir, S.H. and W.Z.A. Mikhail (Eds.). Soil Zoology for Sustainable Development in the 21st Century, Self-Publisher; Cairo, Egypt, PP.397-420.
  9. Fageria, N.K. 2009.The use of nutrients in crop plants.CRC Press, Taylor & Francis Group, LLC.USA. New york.
  10. Glass, K.A., Loeffelholz, J.M., Ford, J.P. and Doyle, M.P. 1992. Fate of E. coli O157:H7 as affected by pH or sodium chloride and in fermented sausage. Applied and Envionment Microbiology 58:2513-2516.
  11. Heitkamp, M.A., Franklin, W. and Cferniglia, C.E. 1988. Microbial metabolism of polycyclic aromatic hydrocarbons: isolation and characterization of a pyrene-degrading bacterium. Applied and Environment Microbiology 54:2549-2555.
  12. Kapoor, K.K., Yadav, K.S., Singh, D.P., Mishra, M.M. and Tauro, P. 1983. Enrichment of compost by Azotobacter and phosphate solubilising microorganisms. Agricultural Wastes  5: 125-133.
  13. Khoshru, B., Sarikhani, M.R. and Aliasgharzad, N. 2017. Inoculation Effect of Some Phosphatic Microbial Fertilizers on Nutritional Indices of Zea mays L. Journal of Water and Soil Science 25: 13-26
  14. Khoshru, B., Sarikhani, M.R., Aliasgharzad, N. and Zare, P. 2015. Assessment the important PGPR features of isolates used in biofertilizers Barvar2, Biosuperphosphate, Supernitroplus and Nitroxin, Applied Soil Research 3: 39-52
  15. Kumar, V. and Narula, N. 1999. Solubilization of inorganic phosphates and growth emergance of wheat as affected by Azotobacter chroococcum. Biology and Fertility of Soils 28: 301-305.
  16. Kumar, V. and Singh, K.P. 2001. Enriching vermicompost by nitrogen fixing and phosphate solubilizing bacteria. Bioresource Technology 76: 173-175.
  17. Lavakush Yadav, J., Verma, J.P., Jaiswal, D.K., and Kumar, A. 2014. Evaluation of PGPR and different concentration of phosphorus level on plant growth, yield and nutrient content of rice (Oryza sativa). Ecological engineering 62: 123–128
  18. Li, L.M., Ding, X.L., Qian, K., Ding Y.Y. and Yin, Z.J. 2011. Effect of microbial consortia on the composting of pig manure. Journal of Animal and Veterinary Advances 10: 1738-1742.
  19. Mclean, E.O. 1982. Soil pH and lime requirement. pp. 199-224. In: A.L Page, R.H. Miller, and D.R. Keeney (eds.) Methods of Soil Analysis. Part 2. Chemical and Microbiological Properties. 2nd ed. Agron. Monogr. 9. ASA and SSSA, Madison, WI.
  20. Naeth, M.A., Bailey, A.W., Chanasyk, D.S. and Pluth, D.J. 1991. Water holding capacity of litter and soil organic matter in mixed prairie and fescue grassland ecosystems of Alberta. Journal of Range management 13-17.
  21. Nelson, L.M. 2004. Plant Growth Promoting Rhizobacteria (PGPR): Prospects for New Inoculants. Plant Management Network.
  22. Piccolo, A. 1996. Humus and soil conservation. In: Piccolo, A. (Ed.), Humic Substances in Terrestrial Ecosystems. Elsevier, Amsterdam, The Netherlands 225–264.
  23. Ravindran, B., Dinesh, S.L., John Kennedy, L. and Sekaran, G. 2008. Vermicomposting of solid waste generated from leather industries using epigeic earthworm eisenia fetida. Applied Biochemical Biotechnology 151: 480–488.
  24. Sarikhani, M.R., Oustan, S., Ebrahimi, M. and Aliasgharzad, N. 2018. Isolation and identification of potassium releasing bacteria in soil and assessment of their ability to release potassium for plants. European Journal of Soil Science.
  25. Sarikhani, M.R., Khoshru, B. and Oustan, S. 2016. Efficiency of Some Bacterial Strains in Potassium Release from Mica and Phosphate Solubilization under in-vitro Conditions. Geomicrobiology Journal 33: 832-838.
  26. Sarikhani, M.R., Malboobi, M.A., and Ebrahimi, M. 2014. Phosphate solubilizing bacteria: Isolation of bacteria and phosphate solubilizing genes, Mechanism and genetics of phosphate solubilization. Journal of Agricultural Biotechnology 6: 76-110.
  27. Shakeela, S., Padder, S.A. and Bhat, Z.A. 2017. Isolation and characterization of plant growth promoting rhizobacteria associated with medicinal plant Picrorhiza Kurroa. Journal of Pharmacognosy and Phytochemistry 6: p.157.
  28. Singh, K. 2009. Microbial and Nutritional Analysis of Vermicompost, Aerobic and Anaerobic Compost. 40 CP Honors Project for Master in Environmental Engineering; Griffith University, Brisbane, Australia; (Supervisors: Dr. Rajiv K. Sinha & Dr. Sunil Heart)
  29. Talashilkar, S.C. 1985. Effect of microbial culture (Azotobacter chroococcum) on humification and enrichment of mechanized compost. Indian journal of agricultural chemistry 22: 193-195.
  30. Turkamani, N. and Alikhani, H. 2008. Comparison of vermicompost from bovine, sheep and poultry fertilizers in different moisture content. Third Congress on Recycling and Use of Renewable Organic Resources in Agriculture. Esfahan. Iran.
  31. Vengadaramana, A. and Jashothan, P.T.J. 2012. Effects of organic fertilizers on the water holding capacity of soil in different terrains of Jaffna peninsula in Sri Lanka.  Journal of Natural Product and Plant Resources 2: 500-503.
  32. Yazdani, M., Bahmanyar, M.A., Pirdashti, H. and Esmaili, M.A. 2009. Effect of Phosphate solubilization microorganisms (PSM) and plant growth promoting rhizobacteria (PGPR) on yield and components of Corn (Zea mays L.). World Academy of Science, Engineering and Technology 37: 90-92.
  33. Wang, P.C., Mori, T., Komori, K., Sasatsu, M., Toda, K. and Ohtake, H. 1989. Isolation and characterization of an Enterobacter cloacae strain that reduces hexavalent chromium under anaerobic conditions. Applied and Environmental Microbiology 55:1665-1669.
  34. Wang, X., Selvam, A., Lau, S.S. and Wong, J.W. 2018. Influence of lime and struvite on microbial community succession and odour emission during food waste composting. Bioresource technology 247: 652-659.